Coding

Part:BBa_K2273110:Design

Designed by: Nina Lautenschlaeger   Group: iGEM17_TU_Dresden   (2017-09-25)


BlaI Repressor of the bla operon derived from Staphylococcus aureus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 66


Design Notes

After codon optimisation the sequence war further modified by adding a Ribosome Binding Site (AGGAGG) and a seven nucleotide spacer upstream of the start codon of the coding sequence.

Prefix with EcoRI, NotI, XbaI, RBS and spacer sequence GAATTCGCGGCCGCTTCTAGAAGGAGGTGTCAAA
Suffix with SpeI, NotI and PstI ACTAGTAGCGGCCGCTGCAGA

Sites of restriction enzymes generating compatible overhangs are indicated by sharing one color. (EcoRI and PstI are marked in blue, NotI in green, XbaI and SpeI in red


Source

This part was chemically synthesized as we needed a codon optimized version of the blaR1 gene from Staphylococcus aureus. The original sequence was taken from the S. aureus N315 genome sequence found in the NCBI database.


References